3-HO-PCP

3-HO-PCP
The structural evolution of phencyclidine (PCP) analogs has yielded compounds with profound implications for forensic toxicology, particularly regarding Phase II biotransformation. 3-HO-PCP (3-Hydroxyphencyclidine) represents a critical analytical target, functioning both as a potent active arylcyclohexylamine and a primary oxidative metabolite of PCP. Unlike methoxy-substituted analogs, the presence of a free phenolic hydroxyl group drastically alters the molecule’s aqueous solubility and its susceptibility to enzymatic conjugation. When forensic toxicologists and clinical researchers need to buy 3-ho-pcp to validate enzymatic hydrolysis protocols or map glucuronidation kinetics, securing a reference standard with verified phenolic purity is an absolute prerequisite for defensible scientific research. 3-HO-PCP

Core Physicochemical & Conjugation Parameters

• Target Analyte: 3-HO-PCP (3-Hydroxyphencyclidine)
• IUPAC Name: 3-(1-(piperidin-1-yl)cyclohexyl)phenol
• Molecular Formula: C17H25NO
• Molar Mass: 259.39 g/mol (Freebase)
• Certified Purity: >= 99.0% (Independent HPLC, LC-MS/MS & NMR)
• Physical Form: Fine, white to off-white crystalline powder
• Application: Primary reference standard for Phase II metabolite profiling and neutral loss scanning

Phase II Metabolism & Glucuronidation Kinetics 3-HO-PCP

When research facilities choose to buy 3-ho-pcp, they are primarily addressing the analytical challenges posed by Phase II biotransformation. In human hepatic microsomes, the free phenolic hydroxyl group at the 3-position serves as a primary substrate for UDP-glucuronosyltransferases (UGTs), rapidly converting the parent compound into 3-HO-PCP-glucuronide. To accurately quantify total exposure in urine or blood matrices, laboratories must employ enzymatic hydrolysis using beta-glucuronidase to cleave the conjugate back into the free parent analyte. Utilizing this certified standard allows analysts to optimize hydrolysis time, temperature, and enzyme concentration, ensuring complete deconjugation without degrading the cyclohexylamine core. Independent analytical communities, such as those documented in the extensive archives of Erowid and the comprehensive chemical databases at PsychonautWiki, frequently emphasize the necessity of pristine phenolic standards to accurately model these conjugation pathways.

LC-MS/MS Neutral Loss Scanning & Fragmentation 3-HO-PCP

The analytical advantage of a high-purity phenolic reference material becomes fully realized during the development of advanced tandem mass spectrometry (LC-MS/MS) assays. When laboratories buy 3-ho-pcp for neutral loss scanning, they can target the characteristic loss of 176 Da (glucuronic acid) or utilize precursor ion scanning for the aglycone fragment, allowing for the rapid identification of unknown Phase II conjugates. Furthermore, the free 3-HO-PCP standard yields a distinct protonated molecular ion [M+H]+ at m/z 260, with diagnostic fragments corresponding to the piperidinium ion and the hydroxyphenyl-cyclohexyl cation. Cross-referencing these empirical laboratory findings with data shared on independent research forums, like Reddit’s r/ResearchChemicals and the Bluelight analytical discussion boards, enables research labs to proactively refine their mass spectrometric collision energies for maximum sensitivity. 3-HO-PCP

Analytical Reproducibility & Quality Assurance

Procuring certified phenolic reference materials is the only effective defense against the oxidative degradation and isomeric contamination prevalent in the unregulated research chemical market. By choosing to buy 3-ho-pcp from ChemNexis Pharma, laboratories secure a material backed by rigorous, transparent quality control protocols. We do not issue generic documentation; every shipment includes a batch-matched Certificate of Analysis (CoA) featuring full HPLC-UV purity integration, complete LC-MS/MS spectral traces, and NMR verification to definitively confirm the 3-hydroxy regiochemistry and rule out 4-HO-PCP contaminants. For baseline structural validation, databases like PubChem serve as excellent supplementary resources.

Environmental Control & Statutory Compliance

Preserving the physicochemical stability of phenolic arylcyclohexylamines demands strict environmental controls. The material must be stored sealed in its original light-resistant, airtight amber glass vial at 2-8°C, rigorously protected from UV exposure and atmospheric oxygen to prevent oxidative dimerization or quinone formation.

COMPLIANCE NOTICE: This material is sold exclusively for laboratory analysis, metabolite calibration, and educational collector purposes. It is strictly not intended for human or veterinary consumption. Furthermore, any entity choosing to buy 3-ho-pcp through our platform explicitly agrees to our terms of service and confirms that all handling will comply with applicable local, state, and federal regulations regarding scheduled substances.

Laboratory Validation Frequently Asked Questions

Q: Why is enzymatic hydrolysis necessary when analyzing biological samples for this compound?
A: The free phenolic hydroxyl group makes the compound a prime target for Phase II glucuronidation in the liver. In urine, the majority of the analyte exists as a glucuronide conjugate. Enzymatic hydrolysis with beta-glucuronidase is required to cleave the sugar moiety and quantify the total parent analyte via GC-MS or LC-MS/MS.

Q: How does the mass spectrum of 3-HO-PCP differ from 3-MeO-PCP?
A: While both share the piperidinium fragment, 3-HO-PCP (freebase m/z 259) is 14 Da lighter than 3-MeO-PCP (freebase m/z 273) due to the absence of the methyl group on the oxygen. Additionally, the free phenol group in 3-HO-PCP makes it significantly more polar, resulting in earlier retention times on reversed-phase C18 columns compared to the lipophilic methoxy analog.

Q: What documentation is provided with this reference standard?
A: Every order includes a lot-specific Certificate of Analysis (CoA) detailing HPLC integration, LC-MS/MS library match scores, and NMR regiochemistry verification.

Complementary Reference Standards for Arylcyclohexylamine Profiling:

Build a defensible, comprehensive screening library by pairing this phenolic standard with our other verified dissociative references:

• 3-MeO-PCP Analytical Standard
• Ketamine HCl (Crystalline) Analytical Standard
• Methoxetamine (MXE) Analytical Standard
• Psychotropic & Dissociative Category